The technological breakthrough of CAS Peter homogenizer in liposome preparation
Liposomes, as the core carriers of nano-drug delivery systems, have become a key technology in anti-tumor drugs, nucleic acid vaccines and functional cosmetics due to their excellent biocompatibility, targeted delivery ability and drug protection properties. CAS Peter has achieved breakthroughs in precise particle size control, improved encapsulation rate and large-scale production in the field of liposome preparation through its independently developed high-pressure homogenizer and microjet technology, providing efficient and stable solutions for the industry.
I. Technical Principle: Multi-effect synergy achieves nanoscale crushing
The CAS Peter homogenizer compresses the material to 800-1200bar through a high-pressure plunger pump, forcing the liquid to form a supersonic jet through diamond microchannels. During this process, the materials undergo three core effects:
Cavitation effect: Under high pressure, liquid vaporizes to form microbubbles, which burst instantaneously and release shock waves, destroying the bilayer structure of liposomes.
Shear effect: High-speed jets generate gradient shear forces within microchannels, tearing liposome vesicles to the nanoscale.
Impact effect: The jet collides with the impact ring to generate an opposing impact force, further refining particles and eliminating agglomeration.
Take the PT-500 production high-pressure homogenizer as an example. It adopts an adjustable pressure valve group design, which can precisely control the particle size from the micrometer level to 85nm in a single cycle. Experimental data show that after three treatments at 1200bar pressure, a semi-transparent state was achieved, indicating a significant optimization of particle size uniformity.
Ii. Process Advantages: Full chain coverage from laboratory to industrialization
Precise control of particle size
The CAS Peter equipment supports particle size range adjustment from 50 to 1000nm, meeting different drug delivery requirements. For instance, in the preparation of lipid nanoparticles (LNP) for mRNA vaccines, the particle size distribution index (PDI) can be compressed to below 0.1 through high-pressure cycles at 800-100bar, ensuring the stability and delivery efficiency of the vaccine.
High encapsulation rate guarantee
The encapsulation rate of liposomes prepared by the traditional thin-film hydration method is usually less than 70%, while the Zhongke Pet homogenizer forces drug molecules into the lipid bilayer gap through high-pressure shear force, increasing the encapsulation rate of hydrophilic drugs to over 92% and the drug loading of hydrophobic drugs to 15wt%.
Iii. Large-scale production capacity
The PTH-20 experimental high-pressure microjet homogenizer has a flow rate exceeding 20L/h, the PT-40 pilot-scale equipment can achieve continuous processing at 48L/h, and the PT-500 pilot-scale equipment can achieve continuous processing at 500L/h, supporting seamless scale-up from gram-level R&D to ton-level industrial production.
The equipment is equipped with a two-stage cooling system, which can keep the processing temperature constant at 4-10℃, avoiding phospholipid oxidation or drug degradation caused by high temperature. Effectively maintain protein activity.
Post time: Aug-16-2025